abstract：AIMS:To select and evaluate an appropriate outer membrane (OM) permeabilizer to use in combination with the highly muralytic bacteriophage endolysin EL188 to inactivate (multi-resistant) Pseudomonas aeruginosa. METHODS AND RESULTS:We tested the combination of endolysin EL188 and several OM permeabilizing compounds on ...

journal_title：Journal of applied microbiology

authors： Briers Y,Walmagh M,Lavigne R

更新日期：2011-03-01 00:00:00

abstract：AIMS:The present work tests the feasibility of the isothermal microcalorimetry method to study the performance of individual lactic acid bacteria during solid-state fermentation in rye sourdough. Another aim was to elucidate the key factors leading to the formation of different microbial consortia in laboratory and ind...

journal_title：Journal of applied microbiology

authors： Mihhalevski A,Sarand I,Viiard E,Salumets A,Paalme T

更新日期：2011-02-01 00:00:00

abstract：AIM:The aim of this study was to characterize the microbial community involved in anaerobic degradation of petroleum hydrocarbon under low- and moderate-temperature conditions. METHODS AND RESULTS:Sulfate-reducing enrichment cultures growing on crude oil and p-xylene were established at low and moderate temperatures. ...

journal_title：Journal of applied microbiology

authors： Higashioka Y,Kojima H,Fukui M

更新日期：2011-01-01 00:00:00

abstract：AIMS:To evaluate the capacity of Oenococcus oeni strains to release aroma compounds from glycosylated precursors by measuring glycosidase activities with both synthetic and natural substrates. METHODS AND RESULTS:Five glycosidase activities were investigated in 47 O. oeni strains using synthetic substrates. This scree...

journal_title：Journal of applied microbiology

authors： Gagné S,Lucas PM,Perello MC,Claisse O,Lonvaud-Funel A,de Revel G

更新日期：2011-01-01 00:00:00

abstract：AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...

journal_title：Journal of applied microbiology

authors： Brown JC,Wang J,Kasman L,Jiang X,Haley-Zitlin V

更新日期：2011-01-01 00:00:00

abstract：AIMS:The aim of this study was to isolate and characterize new bacteriophages that infect a wide range of plant pathogenic Ralstonia solanacearum strains. METHODS AND RESULTS:Fifteen bacteriophages were isolated from pepper, tomato and tobacco plant rhizospheres infected with R. solanacearum. A host specificity analys...

journal_title：Journal of applied microbiology

authors： Murugaiyan S,Bae JY,Wu J,Lee SD,Um HY,Choi HK,Chung E,Lee JH,Lee SW

更新日期：2011-01-01 00:00:00

abstract：AIMS:Validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of Aeromonas hydrophila on the gut development of germ-free Artemia franciscana nauplii. METHODS AND RESULTS:Germ-free Artemia nauplii were cultured using Baker's yeast and dead Aer. hydrophila. Live Aer. hydrophila ...

journal_title：Journal of applied microbiology

authors： Gunasekara RA,Cornillie P,Casteleyn C,De Spiegelaere W,Sorgeloos P,Simoens P,Bossier P,Van den Broeck W

更新日期：2011-01-01 00:00:00

abstract：AIMS:A relative quantification system (RQ-PCR) was used to monitor the correlations between the activity of the nor-1 (=aflD) gene of Aspergillus flavus using real-time PCR in relation to phenotypic aflatoxin B(1) (AFB(1) ) production and populations of A. flavus in stored peanuts at three water activity levels (a(w) ,...

journal_title：Journal of applied microbiology

authors： Abdel-Hadi A,Carter D,Magan N

更新日期：2010-12-01 00:00:00

abstract：AIMS:Dielectric barrier discharge (DBD) plasma is used for sterilization of contaminated inanimate surfaces but seldomly optimized and depends upon the type of organisms and the plasma treatment duration, (net energy deposited) this efficacy varies. The proposed study was designed to see biological responses of one of ...

journal_title：Journal of applied microbiology

authors： Cooper M,Fridman G,Fridman A,Joshi SG

更新日期：2010-12-01 00:00:00

abstract：AIMS:To assess the presence of human adenovirus (HAdV), hepatitis A (HAV) virus and rotavirus A (RV-A) in environmental samples from the Southern region of Brazil and to provide viral contamination data for further epidemiological studies and governmental actions. METHODS AND RESULTS:Water samples from various sources...

journal_title：Journal of applied microbiology

authors： Rigotto C,Victoria M,Moresco V,Kolesnikovas CK,Corrêa AA,Souza DS,Miagostovich MP,Simões CM,Barardi CR

更新日期：2010-12-01 00:00:00

abstract：AIM:Evaluation of the genetic variability of stolbur phytoplasma infecting grapevines, bindweeds and vegetables, collected in different central and southern Italian regions. MATERIALS AND RESULTS:Phytoplasma isolates belonging to stolbur subgroup 16SrXII-A were subjected to molecular characterization by polymerase cha...

journal_title：Journal of applied microbiology

authors： Murolo S,Marcone C,Prota V,Garau R,Foissac X,Romanazzi G

更新日期：2010-12-01 00:00:00

abstract：AIMS:To investigate the effect of diet on the survival of Salmonella in the bovine abomasum. METHODS AND RESULTS:Five fistulated cows were randomly assigned to one of five diets denoted as: (i) 100% grass, (ii) grass + 5·3 kg DM concentrate, (iii) 100% grass silage, (iv) 100% hay and (v) maize/grass silage plus concen...

journal_title：Journal of applied microbiology

authors： Lenahan M,Kelly S,Fanning S,Bolton DJ

更新日期：2010-12-01 00:00:00

abstract：AIMS:Filoviruses are associated with high morbidity and lethality rates in humans, are capable of human-to-human transmission, via infected material such as blood, and are believed to have low infectious doses for humans. Filoviruses are able to infect via the respiratory route and are lethal at very low doses in exper...

journal_title：Journal of applied microbiology

authors： Piercy TJ,Smither SJ,Steward JA,Eastaugh L,Lever MS

更新日期：2010-11-01 00:00:00

abstract：AIMS:The objective of this study was to access APC-effector cell cluster formation in genetically susceptible BALB/c (H-2(d) ) mice infected with highly virulent Moscow strain of ectromelia virus (ECTV-MOS) and estimate of lymphocyte activation based upon expression of CD62L and CD44 molecules. METHODS AND RESULTS:APC...

journal_title：Journal of applied microbiology

authors： Szulc L,Boratynska A,Martyniszyn L,Niemialtowski MG

更新日期：2010-11-01 00:00:00

abstract：AIMS:To investigate the influence of aerobic or vacuum pack storage of beef trimmings on the microbiology, colour and odour of subsequently produced mince. METHODS AND RESULTS:Trimmings stored aerobically for 7 or 10 days and in vacuum packs for 7, 10, 14 or 22 days at 0 or 5°C were minced, stored aerobically at 0 or ...

journal_title：Journal of applied microbiology

authors： Crowley KM,Prendergast DM,Sheridan JJ,McDowell DA

更新日期：2010-10-01 00:00:00

abstract：AIMS:Investigating the effect of perturbations, with relatively low Cu concentrations, on yeast community composition in low-nutrient virgin soil. METHODS AND RESULTS:Culturable soil yeast populations were monitored at an experimental site treated with the fungicide copper oxychloride (10 mg Cu per kg soil). Yeast num...

journal_title：Journal of applied microbiology

authors： Vreulink JM,Stone W,Botha A

更新日期：2010-10-01 00:00:00

abstract：AIMS:To investigate the bactericidal activity of lactoferrin-derived peptides and a new LF-derived peptides chimera (LFchimera) against P. aeruginosa and the influence on virulence factors of P. aeruginosa. METHODS AND RESULTS:Lactoferricin (LFcin) and lactoferrampin (LFampin) are highly bioactive peptides isolated fr...

journal_title：Journal of applied microbiology

authors： Xu G,Xiong W,Hu Q,Zuo P,Shao B,Lan F,Lu X,Xu Y,Xiong S

更新日期：2010-10-01 00:00:00

abstract：AIMS:This study used culture-dependent and culture-independent approaches to characterize bacterial communities in copper plumbing corrosion and to assess biofilm formation and copper resistance of heterotrophic bacteria isolated from copper pipes. METHODS AND RESULTS:Water and copper pipes were collected from a cold-...

journal_title：Journal of applied microbiology

authors： Pavissich JP,Vargas IT,González B,Pastén PA,Pizarro GE

更新日期：2010-09-01 00:00:00

abstract：AIMS:To assess two real-time PCR methods (the Riviere and Qvarnstrom assays) for environmental Acanthamoeba. METHODS AND RESULTS:DNA extracted from Acanthamoeba castellanii taken from water and biofilms of cooling towers was analysed by the Riviere and Qvarnstrom assays. To quantify environmental Acanthamoeba, the cal...

journal_title：Journal of applied microbiology

authors： Chang CW,Wu YC,Ming KW

更新日期：2010-09-01 00:00:00

abstract：AIMS:To monitor seasonal variations in the microbiology of maize silage and to determine whether the risk of fungal spoilage varies during whole-year storage. METHODS AND RESULTS:A continuous survey of 20 maize silage stacks was conducted over a period from three to 11 months after ensiling. Filamentous fungi, yeasts ...

journal_title：Journal of applied microbiology

authors： Storm IM,Kristensen NB,Raun BM,Smedsgaard J,Thrane U

更新日期：2010-09-01 00:00:00

abstract：AIMS:To assess the behaviour of Aspergillus section Nigri IOC 4573 in mango nectar as affected by temperature and pH. METHODS AND RESULTS:A central composite design (2(2)+2*2+3) was used to estimate the influence of temperature (17·2-22·8°C) and pH (3·28-4·7) on A. section Nigri growth (λ, lag time; μ, growth rate). S...

journal_title：Journal of applied microbiology

authors： Silva AR,Sant'Ana AS,Massaguer PR

更新日期：2010-09-01 00:00:00

abstract：AIMS:To assess the biodiversity of a large number of microbial fuel cell (MFC) anodes from a variety of MFC designs, all enriched with domestic wastewater, using a molecular fingerprinting method. METHODS AND RESULTS:We optimized a protocol allowing the rapid characterization of MFC communities using terminal restrict...

journal_title：Journal of applied microbiology

authors： Lefebvre O,Nguyen TT,Al-Mamun A,Chang IS,Ng HY

更新日期：2010-09-01 00:00:00

abstract：AIMS:The aim of this study was to examine the contribution of a five-gene islet (lmo0444 - lmo0448) to the growth of Listeria monocytogenes under suboptimal conditions. METHODS AND RESULTS:Bioinformatics and PCR analyses revealed that a five-gene islet is present in c. half of all L. monocytogenes strains examined (66...

journal_title：Journal of applied microbiology

authors： Ryan S,Begley M,Hill C,Gahan CG

更新日期：2010-09-01 00:00:00

abstract：AIMS:The study was performed to evaluate the safety of whole and RTE vegetables and to investigate the effectiveness of different preventive strategies for the quality assurance of RTE vegetables collected from three Italian production systems. Producer 1, applied a strict system in compliance with GAP- GMP - HACCP, Pr...

journal_title：Journal of applied microbiology

authors： De Giusti M,Aurigemma C,Marinelli L,Tufi D,De Medici D,Di Pasquale S,De Vito C,Boccia A

更新日期：2010-09-01 00:00:00

abstract：AIM:To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus). METHODS AND RESULTS:Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 10(7) CFU g(-1) for 32 days. Ped. acidilactici colonized the intestinal tract and signific...

journal_title：Journal of applied microbiology

authors： Ferguson RM,Merrifield DL,Harper GM,Rawling MD,Mustafa S,Picchietti S,Balcázar JL,Davies SJ

更新日期：2010-09-01 00:00:00

abstract：AIMS:To optimize ethidium monoazide (EMA) coupled with real-time quantitative PCR (qPCR) and to evaluate its environmental applicability on quantifying viable legionellae in water and biofilm of cooling towers and hot water systems. METHODS AND RESULTS:EMA (0.9-45.5 microg ml(-1)) and propidium monoazide (PMA, 0.9 and...

journal_title：Journal of applied microbiology

authors： Chen NT,Chang CW

更新日期：2010-08-01 00:00:00

abstract：AIMS:Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol-acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen-restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also a...

journal_title：Journal of applied microbiology

authors： Nikel PI,Ramirez MC,Pettinari MJ,Méndez BS,Galvagno MA

更新日期：2010-08-01 00:00:00

abstract：:The purpose of this article is to review coculture fermentations in industrial biotechnology. Examples for the advantageous utilization of cocultures instead of single cultivations include the production of bulk chemicals, enzymes, food additives, antimicrobial substances and microbial fuel cells. Coculture fermentati...

journal_title：Journal of applied microbiology

authors： Bader J,Mast-Gerlach E,Popović MK,Bajpai R,Stahl U

更新日期：2010-08-01 00:00:00

abstract：AIMS:A polyphasic approach was used to study the biodiversity bacteria associated with biocorrosion processes, in particular sulfate-reducing bacteria (SRB) and thiosulfate-reducing bacteria (TRB) which are described to be particularly aggressive towards metallic materials, notably via hydrogen sulfide release. METHOD...

journal_title：Journal of applied microbiology

authors： Boudaud N,Coton M,Coton E,Pineau S,Travert J,Amiel C

更新日期：2010-07-01 00:00:00

abstract：AIM:To apply a quantitative reverse-transcription PCR (qRT-PCR) method to determine the total viable count (TVC) on meat samples. METHODS AND RESULTS:Using two sets of primers to target the ribonuclease-P (RNase P) RNA transcripts of Gram-positive and Gram-negative bacteria, standard curves were generated using the Li...

journal_title：Journal of applied microbiology

authors： Dolan A,Burgess CM,Fanning S,Duffy G

更新日期：2010-07-01 00:00:00

abstract：AIM:To identify a DNA sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources. METHODS AND RESULTS:Faecally contaminated poultry litter and soils were used as source material for the development of a quantitative polymerase chain reaction (qPCR) method ta...

journal_title：Journal of applied microbiology

authors： Weidhaas JL,Macbeth TW,Olsen RL,Sadowsky MJ,Norat D,Harwood VJ

更新日期：2010-07-01 00:00:00

abstract：AIMS:To characterize atrazine-degrading potential of bacterial communities enriched from agrochemical factory soil by analysing diversity and organization of catabolic genes. METHODS AND RESULTS:The bacterial communities enriched from three different sites of varying atrazine contamination mineralized 65-80% of (14) C...

journal_title：Journal of applied microbiology

authors： Udiković-Kolić N,Hršak D,Devers M,Klepac-Ceraj V,Petrić I,Martin-Laurent F

更新日期：2010-07-01 00:00:00

abstract：AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...

journal_title：Journal of applied microbiology

authors： Martínez JM,Gómez EV,Pemán J,Cantón E,García MG,del Castillo Agudo L

更新日期：2010-06-01 00:00:00

abstract：AIMS:The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in t...

journal_title：Journal of applied microbiology

authors： Somolinos M,García D,Condón S,Mackey B,Pagán R

更新日期：2010-06-01 00:00:00

abstract：AIMS:To investigate the structural organization and dynamics of exopolysaccharides (EPS) matrix and microcolonies formation by Streptococcus mutans during the biofilm development process. METHODS AND RESULTS:Biofilms of Strep. mutans were formed on saliva-coated hydroxyapatite (sHA) discs in the presence of glucose or...

journal_title：Journal of applied microbiology

authors： Xiao J,Koo H

更新日期：2010-06-01 00:00:00

abstract：AIM:To identify and compare lactic acid bacteria (LAB) isolated from alkaline fermentations of cassava (Manihot esculenta Crantz) leaves, roselle (Hibiscus sabdariffa) and African locust bean (Parkia biglobosa) seeds for production of, respectively, Ntoba Mbodi, Bikalga and Soumbala. METHODS AND RESULTS:A total of 121...

journal_title：Journal of applied microbiology

authors： Ouoba LI,Nyanga-Koumou CA,Parkouda C,Sawadogo H,Kobawila SC,Keleke S,Diawara B,Louembe D,Sutherland JP

更新日期：2010-06-01 00:00:00

abstract：AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contrib...

journal_title：Journal of applied microbiology

authors： Namihira T,Shinzato N,Akamine H,Maekawa H,Matsui T

更新日期：2010-06-01 00:00:00

abstract：AIMS:To develop probiotics for the control of vibriosis caused by Vibrio anguillarum and Vibrio ordalii in finfish. METHODS AND RESULTS:Kocuria SM1, isolated from the digestive tract of rainbow trout, was administered orally to rainbow trout (Oncorhynchus mykiss) for 2 weeks at a dose equivalent to c. 10(8) cells per ...

journal_title：Journal of applied microbiology

authors： Sharifuzzaman SM,Austin B

更新日期：2010-06-01 00:00:00

abstract：AIMS:The isolation of lytic bacteriophage of Vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster Panulirus ornatus. METHODS AND RESULTS:Water samples from discharge channels and grow-out ponds of a prawn farm in northeastern Australia were enriched...

journal_title：Journal of applied microbiology

authors： Crothers-Stomps C,Høj L,Bourne DG,Hall MR,Owens L

更新日期：2010-05-01 00:00:00

abstract：AIM:To investigate the aerobiology of the environment around mechanically ventilated broiler sheds with the aim of understanding dispersion in the surrounding environment. METHODS AND RESULTS:Aerosol samples were collected weekly on four different commercial broiler farms through the cycle of 55 days from 2005 to 2007...

journal_title：Journal of applied microbiology

authors： Chinivasagam HN,Tran T,Maddock L,Gale A,Blackall PJ

更新日期：2010-05-01 00:00:00